Journal: Advanced Science
Article Title: Targeted STING Activation Using Modified Ultrasound‐Responsive Microbubbles Enhances Immune Checkpoint Blockade Against Melanoma
doi: 10.1002/advs.202416596
Figure Lengend Snippet: Formulation and characterization of ncMBs. a ) ncMBs were obtained by conjugating SpeDex‐SH and aCD11b‐SH onto maleimide‐bearing MBs followed by electrostatic binding of cGAMP. b ) Representative Coulter counter measurements (left) and brightfield microscopy (right) of ncMBs show an average size of 2.3 µm. c ) Brightfield microscopy of BMDM, B16‐F10, and D4M.3A cells after incubation with CD11b‐targeted ncMBs validates targeting‐specificity. Scale bar is 50 um. d ) The effect of MUSIC on the viability of BMDMs was determined using the WST‐8 assay 24 h after treatment. Data represent mean ± s.d. and were analyzed by one‐way ANOVA. e ) BMDMs were treated as indicated, followed by western blotting to assess phosphorylation of proteins in the STING pathway.
Article Snippet: Size and concentration were obtained using a Coulter Counter (Beckman Coulter Multisizer 4) and brightfield microscopy (Zeiss Axio A1 upright microscope).
Techniques: Formulation, Binding Assay, Microscopy, Incubation, Western Blot, Phospho-proteomics